Device

Part:BBa_K1438021:Design

Designed by: Johann Bauerfeind   Group: iGEM14_Berlin   (2014-10-10)


Bacterioferritin (BFR) M52H Expression Device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1601
    Illegal XbaI site found at 2891
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 88
    Illegal NheI site found at 745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 88
    Illegal BamHI site found at 145
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1601
    Illegal XbaI site found at 2891
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 88
    Illegal XbaI site found at 1601
    Illegal XbaI site found at 2891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 4264
    Illegal SapI site found at 3181


Design Notes

heme deletion


Source

E. coli Nissle 1917 and Mutagenesis M52H

References